In Vitro Biosynthetic Pathway Investigations of Neuroprotectin D1 (NPD1) and Protectin DX (PDX) by Human 12-Lipoxygenase, 15-Lipoxygenase-1, and 15-Lipoxygenase-2.

ABSTRACT: In this paper, human platelet 12-lipoxygenase [h12-LOX (ALOX12)], human reticulocyte 15-lipoxygenase-1 [h15-LOX-1 (ALOX15)], and human epithelial 15-lipoxygenase-2 [h15-LOX-2 (ALOX15B)] were observed to react with docosahexaenoic acid (DHA) and produce 17S-hydroperoxy-4Z,7Z,10Z,13Z,15E,19Z-docosahexaenoic acid (17S-HpDHA). The k_cat/K_M values with DHA for h12-LOX, h15-LOX-1, and h15-LOX-2 were 12, 0.35, and 0.43 s^-1 μM^-1, respectively, which demonstrate h12-LOX as the most efficient of the three. These values are comparable to their counterpart k_cat/K_M values with arachidonic acid (AA), 14, 0.98, and 0.24 s^-1 μM^-1, respectively.

SUBMITTER: Tsai WC 

PROVIDER: S-EPMC9007043 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature