Gut bacteriobiota and mycobiota are both associated with Day-28 mortality among critically ill patients.

ABSTRACT:

Introduction

Gut microbiota is associated with host characteristics such as age, sex, immune condition or frailty and is thought to be a key player in numerous human diseases. Nevertheless, its association with outcome in critically ill patients has been poorly investigated. The aim of this study is to assess the association between gut microbiota composition and Day-28 mortality in critically ill patients.

Methods

Rectal swab at admission of every patient admitted to intensive care unit (ICU) between October and November 2019 was frozen at - 80 °C. DNA extraction was performed thanks to QIAamp^® PowerFecal^® Pro DNA kit (QIAgen^®). V3-V4 regions of 16SRNA and ITS2 coding genes were amplified by PCR. Sequencing (2x250 bp paired-end) was performe

SUBMITTER: Prevel R 

PROVIDER: S-EPMC9007252 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature