Capturing a rhodopsin receptor signalling cascade across a native membrane.

ABSTRACT: G protein-coupled receptors (GPCRs) are cell-surface receptors that respond to various stimuli to induce signalling pathways across cell membranes. Recent progress has yielded atomic structures of key intermediates^1,2 and roles for lipids in signalling^3,4. However, capturing signalling events of a wild-type receptor in real time, across a native membrane to its downstream effectors, has remained elusive. Here we probe the archetypal class A GPCR, rhodopsin, directly from fragments of native disc membranes using mass spectrometry. We monitor real-time photoconversion of dark-adapted rhodopsin to opsin, delineating retinal isomerization and hydrolysis steps, and further showing that the reaction is significantly slower in its native membrane than in detergent micelles.

SUBMITTER: Chen S 

PROVIDER: S-EPMC9007743 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature