Isolation and culture of murine aortic cells and RNA isolation of aortic intima and media: Rapid and optimized approaches for atherosclerosis research.

ABSTRACT:

Background and aims

Isolation of cellular constituents from the mouse aorta is commonly used for expression or functional analyses in atherosclerosis research. However, current procedures to isolate primary cells are difficult, inefficient, and require separate mice. RNA extraction from aortic intima and media for transcriptomic analysis is also considered difficult with mixed RNA yields. To address these gaps, we provide: 1) a rapid, efficient protocol to isolate and culture diverse cell types concomitantly from the mouse aorta using immunomagnetic cell isolation; and 2) an optimized aortic intimal peeling technique for efficient RNA isolation from the intima and media.

Methods and results

Aortic cells were obtained using an enzymatic solution and different cell types were

SUBMITTER: Chen J 

PROVIDER: S-EPMC9007896 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature