Ultra-deep sequencing data from a liquid biopsy proficiency study demonstrating analytic validity.

ABSTRACT: Recently we reported the accuracy and reproducibility of circulating tumor DNA (ctDNA) assays using a unique set of reference materials, associated analytical framework, and suggested best practices. With the rapid adoption of ctDNA sequencing in precision oncology, it is critical to understand the analytical validity and technical limitations of this cutting-edge and medical-practice-changing technology. The SEQC2 Oncopanel Sequencing Working Group has developed a multi-site, cross-platform study design for evaluating the analytical performance of five industry-leading ctDNA assays. The study used tailor-made reference samples at various levels of input material to assess ctDNA sequencing across 12 participating clinical and research facilities. The generated dataset encompasses multiple

SUBMITTER: Gong B 

PROVIDER: S-EPMC9008010 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature