Cryopreservation of vegetative cells and zygotes of the multicellular volvocine green alga Gonium pectorale.

ABSTRACT:

Background

Colonial and multicellular volvocine green algae have been extensively studied recently in various fields of the biological sciences. However, only one species (Pandorina morum) has been cryopreserved in public culture collections.

Results

Here, we investigated conditions for cryopreservation of the multicellular volvocine alga Gonium pectorale using vegetative colonies or cells and zygotes. Rates of vegetative cell survival in a G. pectorale strain after two-step cooling and freezing in liquid nitrogen were compared between different concentrations (3% and 6%) of the cryoprotectant N,N-dimethylformamide (DMF) and two types of tubes (0.2-mL polymerase chain reaction tubes and 2-mL cryotubes) used for cryopreservation. Among the four conditions investigated, the hi

SUBMITTER: Nozaki H 

PROVIDER: S-EPMC9008917 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature