A comparison of mRNA sequencing (RNA-Seq) library preparation methods for transcriptome analysis.

ABSTRACT:

Background

mRNA sequencing is a powerful technique, which is used to investigate the transcriptome status of a gene of interest, such as its transcription level and splicing variants. Presently, several RNA sequencing (RNA-Seq) methods have been developed; however, the relative advantage of each method has remained unknown. Here we used three commercially available RNA-Seq library preparation kits; the traditional method (TruSeq), in addition to full-length double-stranded cDNA methods (SMARTer and TeloPrime) to investigate the advantages and disadvantages of these three approaches in transcriptome analysis.

Results

We observed that the number of expressed genes detected from the TeloPrime sequencing method was fewer than that obtained using the TruSeq and SMARTer. We also o

SUBMITTER: Ura H 

PROVIDER: S-EPMC9008973 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature