Ex vivo electroretinograms made easy: performing ERGs using 3D printed components.

ABSTRACT:

Key points

Rod and cone photoreceptors convert light into electrochemical signals that are transferred to second order cells, initiating image-forming visual processing. Electroretinograms (ERGs) can detect the associated light-induced extracellular transretinal events, allowing for physiological assessment of cellular activity from morphologically intact retinas. We outline a method for economically configuring a traditional patch-clamp rig for performing high signal-to-noise ex vivo ERGs. We accomplish this by incorporating various 3D printed components and by modifying existing light pathways in a typical patch-clamp rig. This methodology provides an additional set of tools to labs interested in studying the physiological function of neuronal populations in isolated retinal tiss

SUBMITTER: Bonezzi PJ 

PROVIDER: S-EPMC9009099 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature