DO-SRS imaging of diet regulated metabolic activities in Drosophila during aging processes.

ABSTRACT: Lipid metabolism plays crucial roles during aging processes, but how it is regulated by diets and how it interplays with aging still remain unclear. We proposed a new optical imaging platform by integrating heavy water (D₂ O) probing with stimulated Raman scattering (DO-SRS) microscopy, for the first time, to directly visualize and quantify lipid metabolism regulated by different diets and insulin signaling pathway in Drosophila fat body during aging. We found that calorie restriction, low protein diet, and (moderately) high protein and high sucrose diets enhanced lipid turnover in flies at all ages, while (moderately) high fructose and glucose diets only promoted lipid turnover in aged flies. The measured lipid turnover enhancements under diverse diets were due to different mec

SUBMITTER: Li Y 

PROVIDER: S-EPMC9009230 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature