A Platform for Deep Sequence-Activity Mapping and Engineering Antimicrobial Peptides.

ABSTRACT: Developing potent antimicrobials, and platforms for their study and engineering, is critical as antibiotic resistance grows. A high-throughput method to quantify antimicrobial peptide and protein (AMP) activity across a broad continuum would be powerful to elucidate sequence-activity landscapes and identify potent mutants. Yet the complexity of antimicrobial activity has largely constrained the scope and mechanistic bandwidth of AMP variant analysis. We developed a platform to efficiently perform sequence-activity mapping of AMPs via depletion (SAMP-Dep): a bacterial host culture is transformed with an AMP mutant library, induced to intracellularly express AMPs, grown under selective pressure, and deep sequenced to quantify mutant depletion. The slope of mutant growth rate versus in

SUBMITTER: DeJong MP 

PROVIDER: S-EPMC9009525 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature