Contractility analysis of human engineered 3D heart tissues by an automatic tracking technique using a standalone application.

ABSTRACT: The use of Engineered Heart Tissues (EHT) as in vitro model for disease modeling and drug screening has increased, as they provide important insight into the genetic mechanisms, cardiac toxicity or drug responses. Consequently, this has highlighted the need for a standardized, unbiased, robust and automatic way to analyze hallmark physiological features of EHTs. In this study we described and validated a standalone application to analyze physiological features of EHTs in an automatic, robust, and unbiased way, using low computational time. The standalone application "EHT Analysis" contains two analysis modes (automatic and manual) to analyzes the contractile properties and the contraction kinetics of EHTs from high speed bright field videos. As output data, the graphs of displacement, cont

SUBMITTER: Rivera-Arbelaez JM 

PROVIDER: S-EPMC9009597 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature