Transition metal dichalcogenide-based Janus micromotors for on-the-fly Salmonella detection.

ABSTRACT: Janus micromotors encapsulating transition metal dichalcogenides (TMDs) and modified with a rhodamine (RhO)-labeled affinity peptide (RhO-NFMESLPRLGMH) are used here for Salmonella enterica endotoxin detection. The OFF-ON strategy relies on the specific binding of the peptide with the TMDs to induce fluorescence quenching (OFF state); which is next recovered due to selectively binding to the endotoxin (ON state). The increase in the fluorescence of the micromotors can be quantified as a function of the concentration of endotoxin in the sample. The developed strategy was applied to the determination of Salmonella enterica serovar Typhimurium endotoxin with high sensitivity (limits of detection (LODs) of 2.0 µg/mL using MoS₂, and 1.2 µg/mL using WS₂), with quantitative

SUBMITTER: Pacheco M 

PROVIDER: S-EPMC9010330 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature