Fresh tissue procurement and preparation for multicompartment and multimodal analysis of the prostate tumor microenvironment.

ABSTRACT:

Background

Prostatic cancers include a diverse microenvironment of tumor cells, cancer-associated fibroblasts, and immune components. This tumor microenvironment (TME) is a known driving force of tumor survival after treatment, but the standard-of-care tissue freezing or fixation in pathology practice limit the use of available approaches/tools to study the TME's functionality in tumor resistance. Thus, there is a need for approaches that satisfy both clinical and laboratory endpoints for TME study. Here we present methods for clinical case identification, tissue processing, and analytical workflow that are compatible with standard histopathology while enabling molecular and functional interrogation of prostate TME components.

Methods

We first performed a small retrospective

SUBMITTER: Vitek RA 

PROVIDER: S-EPMC9010374 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature