Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates.

ABSTRACT: Cold storage of platelet concentrates (PC) has become attractive due to the reduced risk of bacterial proliferation, but in vivo circulation time of cold-stored platelets is reduced. Ca²⁺ release from storage organelles and higher activity of Ca²⁺ pumps at temperatures < 15 °C triggers cytoskeleton changes. This is suppressed by Mg²⁺ addition, avoiding a shift in Ca²⁺ hemostasis and cytoskeletal alterations. We report on the impact of 2-10 mM Mg²⁺ on cytoskeleton alterations of platelets from PC stored at room temperature (RT) or 4 °C in additive solution (PAS), 30% plasma. Deformation of platelets was assessed by real-time deformability cytometry (RT-DC), a method for biomechanical cell characterization. Deformation was strongly affec

SUBMITTER: Aurich K 

PROVIDER: S-EPMC9010418 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature