Designing a Novel Functional Peptide With Dual Antimicrobial and Anti-inflammatory Activities via in Silico Methods.

ABSTRACT: As spider venom is composed of various bioactive substances, it can be utilized as a platform for discovering future therapeutics. Host defense peptides are great candidates for developing novel antimicrobial agents due to their multifunctional properties. In this study, novel functional peptides were rationally designed to have dual antibacterial and anti-inflammatory activities with high cytocompatibility. Based on a template sequence from the transcriptome of spider Agelena koreana, a series of via in silico analysis were conducted, incorporating web-based machine learning tools along with the alteration of amino acid residues. Two peptides, Ak-N' and Ak-N'm, were designed and were subjected to functional validation. The peptides inhibited gram-negative and gram-positive b

SUBMITTER: Shin MK 

PROVIDER: S-EPMC9010562 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature