Controlling neural territory patterning from pluripotency using a systems developmental biology approach.

ABSTRACT: Successful manufacture of specialized human cells requires process understanding of directed differentiation. Here, we apply high-dimensional Design of Experiments (HD-DoE) methodology to identify critical process parameters (CPPs) that govern neural territory patterning from pluripotency-the first stage toward specification of central nervous system (CNS) cell fates. Using computerized experimental design, 7 developmental signaling pathways were simultaneously perturbed in human pluripotent stem cell culture. Regionally specific genes spanning the anterior-posterior and dorsal-ventral axes of the developing embryo were measured after 3 days and mathematical models describing pathway control were developed using regression analysis. High-dimensional models revealed particular combinations

SUBMITTER: Sears KE 

PROVIDER: S-EPMC9010746 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature