Visualizing tumor angiogenesis and boundary with polygon-scanning multiscale photoacoustic microscopy.

ABSTRACT: Recently, we developed an integrated optical-resolution (OR) and acoustic-resolution (AR) PAM, which has multiscale imaging capability using different resolutions. However, limited by the scanning method, a tradeoff exists between the imaging speed and field of view, which impedes its wider applications. Here, we present an improved multiscale PAM which achieves high-speed wide-field imaging based on a homemade polygon scanner. Encoder trigger mode was proposed to avoid jittering of the polygon scanner during imaging. Distortions caused by polygon scanning were analyzed theoretically and compared with traditional types of distortions in optical-scanning PAM. Then a depth correction method was proposed and verified to compensate for the distortions. System characterization of OR-PAM and AR-

SUBMITTER: Xu Z 

PROVIDER: S-EPMC9010793 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature