Cytotoxic effect of sea anemone pore-forming toxin on K562 chronic myeloid leukemia cells.

ABSTRACT: Chronic myelogenous leukemia (CML) is one of prevalent cancer worldwide. In spite of various designed drugs, chemoresistance remains the main obstacle in cancer cure. Therefore, developing novel strategy for treatment of CML is an urgent need. Fragaceatoxin C (FraC) is novel protein toxin from a sea anemone called actinia fragacea with great impacts against cells by pore formation and disturbing cell membrane integrity. The aim of this study was evaluation of FraC toxin toxicity against K562. The bacteria cells harboring expression‌‌‌‌‌‌‌ vector of FraC were induced by IPTG and purified by Ni²⁺-NTA sepharose affinity chromatography. Then, purified toxin activity was evaluated using RBC hemolytic test. Eventually, evaluation of FraC cytotoxicity and apoptosis were performe

SUBMITTER: Abdoli V 

PROVIDER: S-EPMC9010839 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature