Sonic hedgehog is Essential for Proximal-Distal Outgrowth of the Limb Bud in Salamanders.

ABSTRACT: The developing forelimb has been a foundational model to understand how specified progenitor cells integrate genetic information to produce the tetrapod limb bauplan. Although the reigning hypothesis is that all tetrapods develop limbs in a similar manner, recent work suggests that urodeles have evolved a derived mode of limb dvelopment. Here, we demonstrate through pharmacological and genetic inactivation of Sonic hedgehog (Shh) signaling in axolotls that Shh directs expansion and survival of limb progenitor cells in addition to patterning the limb across the proximodistal and antero-posterior axis. In contrast to inactivation of Shh in mouse or chick embryos where a humerus, radius, and single digit develop, Shh crispant axolotls completely lack forelim

SUBMITTER: Purushothaman S 

PROVIDER: S-EPMC9010949 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature