[Whole-transcriptome sequencing analysis of placental differential miRNA expression profile in Down syndrome].

ABSTRACT:

Objective

To identify new biomarkers and molecular pathogenesis of Down syndrome (DS) by analyzing differentially expressed miRNAs in the placentas and their biological pathways.

Methods

Whole transcriptome sequencing was used to identify the differentially expressed miRNAs in DS (n=3) and normal placental samples (n=3) diagnosed by prenatal diagnosis. The target genes were predicted using miRWalk, Targetscan and miRDB, and GO and KEGG pathway analyses were performed for gene enrichment studies.

Results

We identified a total of 82 differentially expressed miRNAs in the placental tissues of DS, including 29 up-regulated miRNAs (fold change ≥2, P < 0.05) and 15 down-regulated miRNAs (fold change ≥2, P < 0.05), among which 10 miRNAs with relat

SUBMITTER: He J 

PROVIDER: S-EPMC9010987 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature