GeneChip expression profiling identified OLFML2A as a potential therapeutic target in TNBC cells.

ABSTRACT:

Background

An elevated level of olfactomedin-like-2A (OLFML2A) is unfavorable for female breast cancer patients. Patients with a high mRNA level of OLFML2A receive a poor prognosis. Therefore, we speculate that inhibiting the expression of this gene may be beneficial to breast cancer patients. We previously found that silencing the OLFML2A gene by using mRNA interference significantly inhibited proliferation and migration in triple-negative breast cancer (TNBC) cells.

Methods

Cell activity and proliferation were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Celigo analyses. Cell migration and invasion were determined by wound-healing and transwell invasion assays. The mechanism of the inhibition of a small hairpin R

SUBMITTER: Gao X 

PROVIDER: S-EPMC9011253 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature