The activation of SIRT3 by dexmedetomidine mitigates limb ischemia-reperfusion-induced lung injury.

ABSTRACT:

Background

The lung is one of the most sensitive organs, and is vulnerable to injury caused by limb ischemia-reperfusion (LIR). Dexmedetomidine, an anesthetic adjunct, has been shown to have therapeutic effects on lung injury secondary to LIR. This study aimed to investigate the role of dexmedetomidine in ameliorating LIR-induced lung injury in a mouse model of bilateral hind LIR.

Methods

In this study, 75 mice were randomly divided into 5 groups to prepare the LIR model. After the model was established, arterial blood was extracted for blood gas analysis. The pathological changes of lung tissue, lung wet/dry weight ratio, arterial blood gas analysis, detection of myeloperoxidase (MPO) activity, the content of reactive oxygen species (ROS), superoxide dismutase (SOD), glutat

SUBMITTER: Wang L 

PROVIDER: S-EPMC9011293 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature