Effects of SOCS1-overexpressing dendritic cells on Th17- and Treg-related cytokines in COPD mice.

ABSTRACT:

Background

In this study, we established a chronic obstructive pulmonary disease (COPD) model by stimulating mice with cigarette smoke, and observed the effects of dendritic cells (DCs) overexpressing SOCS1 on Th17, Treg and other related cytokines in peripheral blood, bronchoalveolar lavage fluid and lung tissues of COPD mice.

Methods

After successfully transfecting DCs with overexpressing SOCS1 (DC-SOCS1), the mice were injected with DC-SOCS1 (1 × 10⁶), DC-SOCS1 (2 × 10⁶) and immature DCs (1 × 10⁶) via tail vein on days 1 and 7 of COPD fumigation modeling. After day 28 of modeling, the peripheral blood, BALF and lung tissue samples were extracted from the mice, and the changes of DCs, Th17 and Treg cells and related cytokines were detected

SUBMITTER: Liao SX 

PROVIDER: S-EPMC9012034 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature