Microfluidic Squeezing Enables MHC Class I Antigen Presentation by Diverse Immune Cells to Elicit CD8⁺ T Cell Responses with Antitumor Activity.

ABSTRACT: CD8⁺ T cell responses are the foundation of the recent clinical success of immunotherapy in oncologic indications. Although checkpoint inhibitors have enhanced the activity of existing CD8⁺ T cell responses, therapeutic approaches to generate Ag-specific CD8⁺ T cell responses have had limited success. Here, we demonstrate that cytosolic delivery of Ag through microfluidic squeezing enables MHC class I presentation to CD8⁺ T cells by diverse cell types. In murine dendritic cells (DCs), squeezed DCs were ∼1000-fold more potent at eliciting CD8⁺ T cell responses than DCs cross-presenting the same amount of protein Ag. The approach also enabled engineering of less conventional APCs, such as T cells, for effective priming of CD8⁺

SUBMITTER: Booty MG 

PROVIDER: S-EPMC9012083 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature