Sequence analysis and function of mosquito aeCCC2 and Drosophila Ncc83 orthologs.

ABSTRACT: Dipteran insects have genes that code for two different Na⁺-dependent cation-chloride cotransporter (CCC) paralogs. Aedes aegypti aeNKCC1 is an ortholog of Drosophila melanogaster Ncc69, a bumetanide-sensitive Na⁺-K⁺-2Cl^- cotransporter (NKCC). Aedes aegypti aeCCC2 and aeCCC3 are orthologs of Drosophila Ncc83. Prior work suggests that the transport properties of aeCCC2 differ from canonical NKCCs. In particular, Xenopus oocytes expressing aeCCC2 have increased Na⁺-dependent membrane currents compared to controls, whereas NKCCs are electroneutral. Here, we further evaluated the function and localization of aeCCC2 and Ncc83. In oocytes expressing aeCCC2 or Ncc83, membrane potential (V_m) hyperpolarized upon Na⁺ remova

SUBMITTER: Duong PC 

PROVIDER: S-EPMC9012228 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature