Crystal structure of the Ilheus virus helicase: implications for enzyme function and drug design.

ABSTRACT:

Background

The Ilheus virus (ILHV) is an encephalitis associated arthropod-borne flavivirus. It was first identified in Ilheus City in the northeast Brazil before spreading to a wider geographic range. No specific vaccines or drugs are currently available for the treatment of ILHV infections. The ILHV helicase, like other flavivirus helicases, possesses 5'-triphosphatase activity. This allows it to perform ATP hydrolysis to generate energy as well as sustain double-stranded RNA's unwinding during ILHV genome replication. Thus, ILHV helicase is an ideal target for inhibitor design.

Results

We determined the crystal structure of the ILHV helicase at 1.75-Å resolution. We then conducted molecular docking of ATP-Mn²⁺ to the ILHV helicase. Comparisons with related flav

SUBMITTER: Wang DP 

PROVIDER: S-EPMC9012436 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature