CRISPRi for specific inhibition of miRNA clusters and miRNAs with high sequence homology.

ABSTRACT: miRNAs form a class of noncoding RNAs, involved in post-transcriptional regulation of gene expression, broadly studied for their involvement in physiological and pathological context. Inhibition of mature miRNA transcripts, commonly used in miRNA loss-of-function experiments, may not be specific in case of miRNAs with high sequence homology, e.g. miRNAs from the same seed family. Phenotypic effects of miRNA repression might be biased by the repression of highly similar miRNAs. Another challenge is simultaneous inhibition of multiple miRNAs encoded within policistronic clusters, potentially co-regulating common biological processes. To elucidate roles of miRNA clusters and miRNAs with high sequence homology, it is of key importance to selectively repress only the miRNAs of interest. Targeti

SUBMITTER: Drobna-Sledzinska M 

PROVIDER: S-EPMC9012752 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature