Mass recalibration for desorption electrospray ionization mass spectrometry imaging using endogenous reference ions.

ABSTRACT:

Background

Mass spectrometry imaging (MSI) data often consist of tens of thousands of mass spectra collected from a sample surface. During the time necessary to perform a single acquisition, it is likely that uncontrollable factors alter the validity of the initial mass calibration of the instrument, resulting in mass errors of magnitude significantly larger than their theoretical values. This phenomenon has a two-fold detrimental effect: (a) it reduces the ability to interpret the results based on the observed signals, (b) it can affect the quality of the observed signal spatial distributions.

Results

We present a post-acquisition computational method capable of reducing the observed mass drift by up to 60 ppm in biological samples, exploiting the presence of typical molecu

SUBMITTER: Inglese P 

PROVIDER: S-EPMC9013061 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature