IL-2 secretion-based sorting of single T cells using high-throughput microfluidic on-cell cytokine capture.

ABSTRACT: Secreted proteins are critical for the coordination of potent immune defenses, such as in engineered T cell therapies, however, there are few widely accessible approaches to accurately analyze and sort large numbers of cells based on their secretory functions. We report a workflow for the rapid screening and sorting of single individual T cells based on IL-2 secretion accumulated at high concentrations in nanoliter droplets and encoded back onto the secreting cell's surface. In our method, droplets are used solely to partition cells, enabling rapid accumulation of signals onto cell surfaces, and eliminating diffusive crosstalk between neighbors. All downstream sorting leverages conventional high-throughput and readily accessible flow cytometry after the emulsion is disrupted. We achieve mo

SUBMITTER: Dimatteo R 

PROVIDER: S-EPMC9013285 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature