High mRNA expression of POU2F3 in small cell lung cancer cell lines predicts the effect of lurbinectedin.

ABSTRACT:

Background

Small cell lung cancer (SCLC) is a progressive disease with a poor prognosis. Recently, a method to classify SCLC by the expression status of four transcription factors, ASCL1, NEUROD1, POU2F3, and YAP1, was proposed. Here, we investigated the potential relationships between expression of these four transcription factors and the effect of lurbinectedin.

Methods

mRNA and protein expression of ASCL1, NEUROD1, POU2F3, and YAP1 were quantified in eight SCLC cell lines and analyzed for potential correlations with drug sensitivity. In addition, ASCL1, NEUROD1, POU2F3, and YAP1 expression were evaluated in 105 resected cases of high-grade neuroendocrine carcinoma of the lung, including 59 resected cases of SCLC.

Results

Based on the results of qRT-PCR and western

SUBMITTER: Matsui S 

PROVIDER: S-EPMC9013643 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature