Grouping of chemicals into mode of action classes by automated effect pattern analysis using the zebrafish embryo toxicity test.

ABSTRACT: A central element of high throughput screens for chemical effect assessment using zebrafish is the assessment and quantification of phenotypic changes. By application of an automated and more unbiased analysis of these changes using image analysis, patterns of phenotypes may be associated with the mode of action (MoA) of the exposure chemical. The aim of our study was to explore to what extent compounds can be grouped according to their anticipated toxicological or pharmacological mode of action using an automated quantitative multi-endpoint zebrafish test. Chemical-response signatures for 30 endpoints, covering phenotypic and functional features, were generated for 25 chemicals assigned to 8 broad MoA classes. Unsupervised clustering of the profiling data demonstrated that chemicals were

SUBMITTER: Teixido E 

PROVIDER: S-EPMC9013687 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature