YTHDF1 Negatively Regulates Treponema pallidum-Induced Inflammation in THP-1 Macrophages by Promoting SOCS3 Translation in an m6A-Dependent Manner.

ABSTRACT:

Background

Previous studies have confirmed that the bacterium Treponema pallidum (TP) or its proteins provide signals to macrophages that induce an inflammatory response; however, little is known about the negative regulation of this macrophage-mediated inflammatory response during syphilis infection or the underlying mechanism. Recent evidence suggests the role of the RNA modification, N⁶-adenosine methylation (m6A), in regulating the inflammatory response and pathogen-host cell interactions. Therefore, we hypothesized that m6A plays a role in the regulation of the inflammatory response in macrophages exposed to TP.

Methods

We first assessed m6A levels in TP-infected macrophages differentiated from the human monocyte cell line THP-1. The binding and intera

SUBMITTER: Li Z 

PROVIDER: S-EPMC9013966 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature