Spatial and single-cell transcriptome analysis reveals changes in gene expression in response to drug perturbation in rat kidney.

ABSTRACT: The kidney is a complex organ that consists of various types of cells. It is occasionally difficult to resolve molecular alterations and possible perturbations that the kidney experiences due to drug-induced damage. In this study, we performed spatial and single-cell transcriptome analysis of rat kidneys and constructed a precise rat renal cell atlas with spatial information. Using the constructed catalogue, we were able to characterize cells of several minor populations, such as macula densa or juxtaglomerular cells. Further inspection of the spatial gene expression data allowed us to identify the upregulation of genes involved in the renin regulating pathway in losartan-treated populations. Losartan is an angiotensin II receptor antagonist drug, and the observed upregulation of the renin

SUBMITTER: Onoda N 

PROVIDER: S-EPMC9014450 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature