Development of Novel ¹⁹¹Pt-Labeled Hoechst33258: ¹⁹¹Pt Is More Suitable than ¹¹¹In for Targeting DNA.

ABSTRACT: This study aims to establish new labeling methods for no-carrier-added radio-Pt (¹⁹¹Pt) and to evaluate the in vitro properties of ¹⁹¹Pt-labeled agents compared with those of agents labeled with the common emitter ¹¹¹In. ¹⁹¹Pt was complexed with the DNA-targeting dye Hoechst33258 via diethylenetriaminepentaacetic acid (DTPA) or the sulfur-containing amino acid cysteine (Cys). The intranuclear fractions of ¹⁹¹Pt- and ¹¹¹In-labeled Hoechst33258 were comparable, indicating that the labeling for ¹⁹¹Pt via DTPA or Cys and the labeling for ¹¹¹In via DTPA worked equally well. ¹⁹¹Pt showed a DNA-binding/cellular uptake ratio of more than 1 order of magnitude greater than that of ¹¹¹In. [

SUBMITTER: Obata H 

PROVIDER: S-EPMC9014856 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature