Multiplexed Cross-Linking with Isobaric Quantitative Protein Interaction Reporter Technology.

ABSTRACT: Chemical cross-linking with mass spectrometry (XL-MS) has emerged as a useful technique for interrogating protein structures and interactions. When combined with quantitative proteomics strategies, protein conformational and interaction dynamics can be probed. Quantitative XL-MS has been demonstrated with the use of stable isotopes incorporated metabolically or into the cross-linker molecules. Isotope-labeled cross-linkers have primarily utilized deuterium and rely on MS1-based quantitation of precursor ion extracted ion chromatograms. Recently the development and application of isobaric quantitative protein interaction reporter (iqPIR) cross-linkers were reported, which utilize ¹³C and ¹⁵N isotope labels. Quantitation is accomplished using relative fragment ion isoto

SUBMITTER: Chavez JD 

PROVIDER: S-EPMC9014865 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature