Regulation of liver subcellular architecture controls metabolic homeostasis.

ABSTRACT: Cells display complex intracellular organization by compartmentalization of metabolic processes into organelles, yet the resolution of these structures in the native tissue context and their functional consequences are not well understood. Here we resolved the three-dimensional structural organization of organelles in large (more than 2.8 × 10⁵ µm³) volumes of intact liver tissue (15 partial or full hepatocytes per condition) at high resolution (8 nm isotropic pixel size) using enhanced focused ion beam scanning electron microscopy^1,2 imaging followed by deep-learning-based automated image segmentation and 3D reconstruction. We also performed a comparative analysis of subcellular structures in liver tissue of lean and obese mice and found substantial altera

SUBMITTER: Parlakgul G 

PROVIDER: S-EPMC9014868 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature