The Extended Hadamard Transform: Sensitivity-Enhanced NMR Experiments Among Labile and Non-Labile ¹ Hs of SARS-CoV-2-derived RNAs.

ABSTRACT: Hadamard encoded saturation transfer can significantly improve the efficiency of NOE-based NMR correlations from labile protons in proteins, glycans and RNAs, increasing the sensitivity of cross-peaks by an order of magnitude and shortening experimental times by ≥100-fold. These schemes, however, fail when tackling correlations within a pool of labile protons - for instance imino-imino correlations in RNAs or amide-amide correlations in proteins. Here we analyze the origin of the artifacts appearing in these experiments and propose a way to obtain artifact-free correlations both within the labile pool as well as between labile and non-labile ¹ Hs, while still enjoying the gains arising from Hadamard encoding and solvent repolarizations. The principles required for implementing w

SUBMITTER: Kim J 

PROVIDER: S-EPMC9015374 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature