Chronic Sleep Deprivation Impaired Bone Formation in Growing Rats and Down-Regulated PI3K/AKT Signaling in Bone Tissues.

ABSTRACT:

Background

This study aimed to assess the effects of chronic sleep deprivation (CSD) on bone metabolism in growing rats and the likely underlying mechanism.

Methods

Twenty 5-week-old male Wistar rats and randomly divided into the CSD and normal control (NC) groups after one-week acclimatization. After a 6-week intervention of sleep deprivation, the distal femurs of both groups were harvested for micro-computed tomography scans and histological analysis. Meanwhile, the femur tissues were measured the mRNA and protein expression via RNA sequencing and immunohistochemical analysis. Serum bone turnover markers were evaluated at 0, 2, 4, and 6 weeks.

Results

CSD impaired the bone growth, showing an imbalance of bone turnover status, dysphasia in the metaphysis growth plate

SUBMITTER: Duan X 

PROVIDER: S-EPMC9015811 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature