Microfluidic circulating reactor system for sensitive and automated duplex-specific nuclease-mediated microRNA detection.

ABSTRACT: Duplex-specific nuclease signal amplification (DSNSA) is a promising microRNA (miRNA) quantification strategy. However, existing DSNSA based miRNA detection methods suffer from costly chemical consumptions and require laborious multi-step sample pretreatment that are prone to sample loss and contamination, including total RNA extraction and enrichment. To address these problems, herein we devised a pneumatically automated microfluidic reactor device that integrates both analyte extraction/enrichment and DSNSA-mediated miRNA detection in one streamlined analysis workflow. Two flow circulation strategies were investigated to determine the effects of flow conditions on the kinetics of on-chip DSNSA reaction in a bead-packed microreactor. With the optimized workflow, we demonstrated rapid, rob

SUBMITTER: Zhou X 

PROVIDER: S-EPMC9015856 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature