Dataset Information

Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application.

ABSTRACT: This study focused on the optimization of ultrasound-assisted compound enzyme extraction for polysaccharides (RTPs) from Radix trichosanthis by orthogonal experiment and response surface methodology, and then its extraction kinetics model and antihyperlipidemic activities were studied. The optimum extraction process was as follows: cellulase-1.0%, papain-1.0%, pectase-0.5%, pH-5, extraction temperature-50°C, and liquid-to-solid ratio-30 mL/g; prediction value of RTPs was 7.54%; the experimental yield of RTPs was 7.22%, while 50 minutes was optimized in Weibull kinetics model. Then high-dose groups of RTP extract could reduce the TC, TG, and LDL-C levels and increase the level of HDL-C in high-fat mice, with the ability to lower the MDA content and enhance SOD level.

SUBMITTER: Chen F

PROVIDER: S-EPMC9015872 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Extraction of <i>Radix trichosanthis</i> Polysaccharides for Potential Antihyperlipidemic Application.

Chen Fujia F Xu Yilin Y Ding Nana N Li Hongyan H Li Tongbiao T Liu Fengyun F Liang Mengxue M Song Li L Liu Junhe J Li Enzhong E Liu Jiayang J

BioMed research international 20220411

This study focused on the optimization of ultrasound-assisted compound enzyme extraction for polysaccharides (RTPs) from <i>Radix trichosanthis</i> by orthogonal experiment and response surface methodology, and then its extraction kinetics model and antihyperlipidemic activities were studied. The optimum extraction process was as follows: cellulase-1.0%, papain-1.0%, pectase-0.5%, pH-5, extraction temperature-50°C, and liquid-to-solid ratio-30 mL/g; prediction value of RTPs was 7.54%; the experi ...[more]

PMID: 35445134

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Project description:Adansonia digitata L. is an African tree commonly called baobab. This tree is effectively used in traditional medicine to treat cardiovascular disorders. Hyperlipidemia is a well-known cardiovascular risk factor associated with the increased incidence of mortality worldwide. This study aimed to demonstrate the mechanism of baobab polyphenols in the activities of hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase and pancreatic lipase as lipid metabolic enzymes. Molecular docking and an incentive for drug design showed that all the polyphenols in baobab bound to the proteins with higher affinity and a lower binding energy compared with simvastatin as the positive control (ΔG: from -5.5 kcal/mol to -6.5 kcal/mol). The same polyphenols exhibited a considerable binding affinity to pancreatic lipase (ΔG: from -7.5 kcal/mol to -9.8 kcal/mol) in comparison with the control and HMG-CoA reductase. Quercetin showed the best docking score from the selected Baobab polyphenols (ΔG = -9.8 kcal/mol). The root mean square deviation (RMSD) results indicated that stable epicatechin and quercetin complexes were demonstrated with HMG-CoA reductase, and other less stable complexes were developed using rutin and chlorogenic acid. Moreover, the analysis of the root mean square fluctuation (RMSF) simulation results was consistent with that of the RMSD. The RMSF value for all the baobab polyphenols, including the crystal control ligand, was kept between 0.80 and 8.00 Å, similarly to simvastatin, and less than 4.8 Å for pancreatic lipase. Chlorogenic acid, quercetin, epicatechin, and rutin had negative ΔG binding scores from highest to lowest. The same ligands displayed more negative ΔG binding scores than those observed in HMG-CoA reductase and crystal control ligand (methoxyundecyl phosphinic acid) in their simulation with pancreatic lipase. In conclusion, baobab polyphenols interact with HMG-CoA reductase and pancreatic lipase to inhibit their substrate binding and block their activity.

Dataset Information

Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application.

Publications

Extraction of <i>Radix trichosanthis</i> Polysaccharides for Potential Antihyperlipidemic Application.

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