Translational imaging of the fibroblast activation protein (FAP) using the new ligand [⁶⁸Ga]Ga-OncoFAP-DOTAGA.

ABSTRACT:

Purpose

The fibroblast activation protein (FAP) is an emerging target for molecular imaging and therapy in cancer. OncoFAP is a novel small organic ligand for FAP with very high affinity. In this translational study, we establish [⁶⁸Ga]Ga-OncoFAP-DOTAGA (⁶⁸Ga-OncoFAP) radiolabeling, benchmark its properties in preclinical imaging, and evaluate its application in clinical PET scanning.

Methods

⁶⁸Ga-OncoFAP was synthesized in a cassette-based fully automated labeling module. Lipophilicity, affinity, and serum stability of ⁶⁸Ga-OncoFAP were assessed by determining logD_7.4, IC₅₀ values, and radiochemical purity. ⁶⁸Ga-OncoFAP tumor uptake and imaging properties were assessed in preclinical dynamic PET/MR

SUBMITTER: Backhaus P 

PROVIDER: S-EPMC9016025 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature