Ex vivo propagation in a novel 3D high-throughput co-culture system for multiple myeloma.

ABSTRACT:

Purpose

Multiple myeloma (MM) remains an incurable hematologic malignancy which ultimately develops drug resistance and evades treatment. Despite substantial therapeutic advances over the past years, the clinical failure rate of preclinically promising anti-MM drugs remains substantial. More realistic in vitro models are thus required to better predict clinical efficacy of a preclinically active compound.

Methods

Here, we report on the establishment of a conical agarose 3D co-culture platform for the preclinical propagation of primary MM cells ex vivo. Cell growth was compared to yet established 2D and liquid overlay systems. MM cell lines (MMCL: RPMI-8226, U266, OPM-2) and primary patient specimens were tested. Drug sensitivity was examined by exploring the cytotoxic effect

SUBMITTER: Waldschmidt JM 

PROVIDER: S-EPMC9016043 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature