The Highly Repeat-Diverse (Peri) Centromeres of White Lupin (Lupinus albus L.).

ABSTRACT: Plant genomes are known to be mainly composed of repetitive DNA sequences. Regardless of the non-genic function of these sequences, they are important for chromosome structure and stability during cell-cycle. Based on the recent available whole-genome assembly of white lupin (Lupinus albus L.; WL), we have in silico annotated and in situ mapped the main classes of DNA repeats identified with RepeatExplorer. A highly diverse and an abundance of satellite DNAs were found representing more than 10 families, where three of them were highly associated with CENH3-immunoprecipitated chromatin. Applying a strategy of several re-hybridization steps with different combinations of satDNA, rDNA, and LTR-RTs probes, we were able to construct a repeat-based chromosome map for the id

SUBMITTER: Marques A 

PROVIDER: S-EPMC9016224 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature