Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions.

ABSTRACT: We report biocompatible materials for cryostorage of unstable proteins such as cancer-related enzyme, N-acetylglucosaminyltransferase-V (GnT-V). GnT-V activity and the amount of protein after freezing were better retained in synthetic zwitterion solutions than in the glycerol solution. This study highlights the potential utility of synthetic zwitterions as novel cryoprotectants.

SUBMITTER: Hirata T 

PROVIDER: S-EPMC9016413 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature