Modulating the Cellular Immune Response of Oligonucleotides by Brush Polymer-Assisted Compaction.

ABSTRACT: Unwanted stimulation of the innate immune system by foreign nucleic acids has been one of the major barriers preventing bioactive sequences from reaching market. Foreign nucleic acids can be recognized by multiple pattern recognition receptors (PRRs), which trigger a signaling cascade to activate host defense systems, leading to a range of side effects. This study demonstrates that polyethylene glycol (PEG)-modified DNA strands can greatly reduce the activation of the innate immune system, and the extent of reduction is dependent upon polymer architecture. Highly branched brushes with long PEG side chains achieve the best suppression by blocking PRR interactions via a local steric effect. Interestingly, the brush polymer creates little barrier toward DNA-DNA interaction. Quantification of

SUBMITTER: Cao X 

PROVIDER: S-EPMC9016755 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature