Putative Phenotypically Neutral Genomic Insertion Points in Prokaryotes.

ABSTRACT: The barriers to effective genome editing in diverse prokaryotic organisms have been falling at an accelerated rate. As editing becomes easier in more organisms, quickly identifying genomic locations to insert new genetic functions without disrupting organism fitness becomes increasingly useful. When the insertion is noncoding DNA for applications such as information storage or barcoding, a neutral insertion point can be especially important. Here we describe an approach to identify putatively neutral insertion sites in prokaryotes. An algorithm (targetFinder) finds convergently transcribed genes with gap sizes within a specified range, and looks for annotations within the gaps. We report putative editing targets for 10 common synthetic biology chassis organisms, including coverage of avail

SUBMITTER: Bernhards CB 

PROVIDER: S-EPMC9016761 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature