Bioinformatic Design of Dendritic Cell-Specific Synthetic Promoters.

ABSTRACT: Next-generation DNA vectors for cancer immunotherapies and vaccine development require promoters eliciting predefined transcriptional activities specific to target cell types, such as dendritic cells (DCs), which underpin immune response. In this study, we describe the de novo design of DC-specific synthetic promoters via in silico assembly of cis-transcription factor response elements (TFREs) that harness the DC transcriptional landscape. Using computational genome mining approaches, candidate TFREs were identified within promoter sequences of highly expressed DC-specific genes or those exhibiting an upregulated expression during DC maturation. Individual TFREs were then screened in vitro in a target DC line and off-target cell lines derived from skeletal muscl

SUBMITTER: Johnson AO 

PROVIDER: S-EPMC9016764 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature