Rapid electrochemical detection of MiRNA-21 facilitated by the excellent catalytic ability of Pt@CeO₂ nanospheres.

ABSTRACT: Pt@CeO₂ nanospheres (NSs) were first synthesized by simply mixing Ce(NO₃)₃ and K₂PtCl₄ under the protection of pure argon at 70 °C for 1 h, which exhibited excellent catalytic ability toward hydrogen peroxide (H₂O₂). An electrochemical biosensor was successfully developed using Pt@CeO₂ NSs as a capture probe for the ultra-sensitive and fast detection of miRNA-21, a new type of biomarker for disease diagnostics, especially for cancer. During the step-by-step construction process of the RNA sensor, Pt@CeO₂ NSs were functionalized with streptavidin (SA) to obtain SA-Pt@CeO₂ NSs through amide bonds. Gold nanoparticles (Au NPs) were electrodeposited on the surface of the glassy carbon e

SUBMITTER: Chen P 

PROVIDER: S-EPMC9016849 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature