Dataset Information

Potato StLecRK-IV.1 negatively regulates late blight resistance by affecting the stability of a positive regulator StTET8.

ABSTRACT: Plant receptor-like kinases (RLKs) regulate many processes in plants. Many RLKs perform significant roles in plant immunity. Lectin receptor-like kinases (LecRLKs) are a large family of RLKs. However, the function of most of LecRLKs is poorly understood. In this study, we show that a potato LecRLK, StLecRK-IV.1, is involved in plant immunity against Phytophthora infestans. As a negative regulator of immunity, StLecRK-IV.1 is down-regulated by P. infestans and activated by abscisic acid (ABA). The transient expression of StLecRK-IV.1 in Nicotiana benthamiana enhanced P. infestans leaf colonization significantly. In contrast, the disease lesion size caused by P. infestans was reduced in Virus-induced gene silencing (VIGS) of StLecRK-IV.1 orthologue in N. benthamiana, NbLecRK-IV.1, as well as in potato plants with stable RNA interference of StLecRK-IV.1. Tetraspanin-8 (StTET8) was identified to be interacting with StLecRK-IV.1 using a membrane yeast-2-hybrid system, which was further verified by co-immunoprecipitation, a luciferase complementation assay, and a bimolecular fluorescence complementary (BiFC) test. StTET8 is a positive immune regulator that restrains P. infestans infection. The co-expression of StLecRK-IV.1 with StTET8 antagonized the positive roles of StTET8 against P. infestans. Moreover, the co-expression of StTET8 with StLecRK-IV.1 affected the stability of StTET8, which was confirmed by a Western blot assay and confocal assay. Taken together, our work firstly revealed that a potato L-type Lectin RLK, StLecRK-IV.1, negatively regulates plant immunity by targeting a positive regulator, StTET8, through affecting its stability.

SUBMITTER: Guo L

PROVIDER: S-EPMC9016858 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

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Potato StLecRK-IV.1 negatively regulates late blight resistance by affecting the stability of a positive regulator StTET8.

Guo Lei L Qi Yetong Y Mu Yang Y Zhou Jing J Lu Wenhe W Tian Zhendong Z

Horticulture research 20220211

Plant receptor-like kinases (RLKs) regulate many processes in plants. Many RLKs perform significant roles in plant immunity. Lectin receptor-like kinases (LecRLKs) are a large family of RLKs. However, the function of most of LecRLKs is poorly understood. In this study, we show that a potato LecRLK, StLecRK-IV.1, is involved in plant immunity against Phytophthora infestans. As a negative regulator of immunity, StLecRK-IV.1 is down-regulated by P. infestans and activated by abscisic acid (ABA). Th ...[more]

PMID: 35147183

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Project description:Late blight (LB) caused by the oomycete Phytophthora infestans (Mont.) de Bary is the greatest threat to potato production worldwide. Current potato breeding for LB resistance heavily depends on the introduction of new genes for resistance to P. infestans (Rpi genes). Such genes have been discovered in highly diverse wild, primitive, and cultivated species of tuber-bearing potatoes (Solanum L. section Petota Dumort.) and introgressed into the elite potato cultivars by hybridization and transgenic complementation. Unfortunately, even the most resistant potato varieties have been overcome by LB due to the arrival of new pathogen strains and their rapid evolution. Therefore, novel sources for germplasm enhancement comprising the broad-spectrum Rpi genes are in high demand with breeders who aim to provide durable LB resistance. The Genbank of the N.I. Vavilov Institute of Plant Genetic Resources (VIR) in St. Petersburg harbors one of the world's largest collections of potato and potato relatives. In this study, LB resistance was evaluated in a core selection representing 20 species of seven Petota series according to the Hawkes (1990) classification: Bulbocastana (Rydb.) Hawkes, Demissa Buk., Longipedicellata Buk., Maglia Bitt., Pinnatisecta (Rydb.) Hawkes, Tuberosa (Rydb.) Hawkes (wild and cultivated species), and Yungasensa Corr. LB resistance was assessed in 96 accessions representing 18 species in the laboratory test with detached leaves using a highly virulent and aggressive isolate of P. infestans. The Petota species notably differed in their LB resistance: S. bulbocastanum Dun., S. demissum Lindl., S. cardiophyllum Lindl., and S. berthaultii Hawkes stood out at a high frequency of resistant accessions (7-9 points on a 9-point scale). Well-established specific SCAR markers of ten Rpi genes-Rpi-R1, Rpi-R2/Rpi-blb3, Rpi-R3a, Rpi-R3b, Rpi-R8, Rpi-blb1/Rpi-sto1, Rpi-blb2, and Rpi-vnt1-were used to mine 117 accessions representing 20 species from seven Petota series. In particular, our evidence confirmed the diverse Rpi gene location in two American continents. The structural homologs of the Rpi-R2, Rpi-R3a, Rpi-R3b, and Rpi-R8 genes were found in the North American species other than S. demissum, the species that was the original source of these genes for early potato breeding, and in some cases, in the South American Tuberosa species. The Rpi-blb1/Rpi-sto1 orthologs from S. bulbocastanum and S. stoloniferum Schlechtd et Bché were restricted to genome B in the Mesoamerican series Bulbocastana, Pinnatisecta, and Longipedicellata. The structural homologs of the Rpi-vnt1 gene that were initially identified in the South American species S. venturii Hawkes and Hjert. were reported, for the first time, in the North American series of Petota species.

Dataset Information

Potato StLecRK-IV.1 negatively regulates late blight resistance by affecting the stability of a positive regulator StTET8.

Publications

Potato StLecRK-IV.1 negatively regulates late blight resistance by affecting the stability of a positive regulator StTET8.

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