Fluorescent labeling of genomic loci in Drosophila imaginal discs with heterologous DNA-binding proteins.

ABSTRACT: Using the Drosophila melanogaster Hox gene Ultrabithorax (Ubx) as an example, we demonstrate the use of three heterologous DNA-binding protein systems-LacI/LacO, ParB1/ParS1, and ParB2/ParS2-to label genomic loci in imaginal discs with the insertion of a small DNA tag. We compare each system, considering the impact of labeling in genomic regions (1) inside versus outside of a transcribed gene body and (2) with varying chromatin accessibility. We demonstrate the value of this system by interrogating the relationship between gene expression level and enhancer-promoter distance, as well as inter-allelic distance at the Ubx locus. We find that the distance between an essential intronic cis-regulatory element, anterobithorax (abx), and the promot

SUBMITTER: Delker RK 

PROVIDER: S-EPMC9017127 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature